H2N2V1, Main, Exploration, bibRecord, 001A39

[Evaluation of a rapid enzyme immunoassay membrane test for diagnosis of influenza A virus infection].

Identifieur interne : 001A39 ( Main/Exploration ); précédent : 001A38; suivant : 001A40

[Evaluation of a rapid enzyme immunoassay membrane test for diagnosis of influenza A virus infection].

Auteurs : H. Shimizu ; S. Watanabe ; C. Kawakami ; Y. Hirai ; K. Kimura ; N. Sugaya ; M. Imai

Source :

Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases [ 0387-5911 ] ; 1998.

RBID : pubmed:9780586

Descripteurs français

KwdFr :
- Antigènes viraux (analyse), Enfant, Grippe humaine (diagnostic), Humains, Sensibilité et spécificité, Test ELISA (), Virus de la grippe A (immunologie).
MESH :
- analyse : Antigènes viraux.
- diagnostic : Grippe humaine.
- immunologie : Virus de la grippe A.
- Enfant, Humains, Sensibilité et spécificité, Test ELISA.

English descriptors

KwdEn :
- Antigens, Viral (analysis), Child, Enzyme-Linked Immunosorbent Assay (methods), Humans, Influenza A virus (immunology), Influenza, Human (diagnosis), Sensitivity and Specificity.
MESH :
- chemical , analysis : Antigens, Viral.
- diagnosis : Influenza, Human.
- immunology : Influenza A virus.
- methods : Enzyme-Linked Immunosorbent Assay.
- Child, Humans, Sensitivity and Specificity.

Abstract

A rapid enzyme immunoassay membrane test, Directigen Flu A (Becton Dickinson, USA), was evaluated by using virus isolates and clinical specimens. The reference laboratory diagnosis was based on the results of virus isolation. Directigen Flu A was reactive for all subtypes of human influenza A viruses, including reference strains of H1N1, H2N2 and H3N2. Moreover, H5N1 (Hongkong/156/97) was also detected by this kit. No cross reactivity was detected with other respiratory viruses. Directigen Flu A showed positive reaction with the solution containing influenza A virus of 2.4 x 10(3) pfu/assay. The rapid test demonstrated 77.9% sensitivity and 98.4% specificity for testing of throat swabs from children with respiratory symptoms. It showed higher sensitivity and specificity (92.1% and 100%) for testing of nasopharyngeal aspirates. Directigen Flu A should be useful for the rapid diagnosis of influenza A virus infection.

DOI: 10.11150/kansenshogakuzasshi1970.72.827
PubMed: 9780586

Affiliations:

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<front><div type="abstract" xml:lang="en">A rapid enzyme immunoassay membrane test, Directigen Flu A (Becton Dickinson, USA), was evaluated by using virus isolates and clinical specimens. The reference laboratory diagnosis was based on the results of virus isolation. Directigen Flu A was reactive for all subtypes of human influenza A viruses, including reference strains of H1N1, H2N2 and H3N2. Moreover, H5N1 (Hongkong/156/97) was also detected by this kit. No cross reactivity was detected with other respiratory viruses. Directigen Flu A showed positive reaction with the solution containing influenza A virus of 2.4 x 10(3) pfu/assay. The rapid test demonstrated 77.9% sensitivity and 98.4% specificity for testing of throat swabs from children with respiratory symptoms. It showed higher sensitivity and specificity (92.1% and 100%) for testing of nasopharyngeal aspirates. Directigen Flu A should be useful for the rapid diagnosis of influenza A virus infection.</div>
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[Evaluation of a rapid enzyme immunoassay membrane test for diagnosis of influenza A virus infection].

[Evaluation of a rapid enzyme immunoassay membrane test for diagnosis of influenza A virus infection].

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

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